General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CNR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0959E2CP0M)
The PathHunter CNR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CNR1 and EA-tagged β-Arrestin-2. Activation of CNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD5 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0094E2CP2M)
The cAMP Hunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD5, a naturally Gs coupled GPCR. Upon activation of DRD5, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD2S (Short Isoform) CHO-K1 GPCR Assay, 10pl Kit. (95-0084E2CP2L)
The cAMP Hunter DRD2S eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD2S GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD2S, a naturally Gi/Go coupled GPCR. Upon activation of DRD2S, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CMKLR1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0080E2CP2L)
The cAMP Hunter CMKLR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. chemerin) based activation of CMKLR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CMKLR1, a naturally Gi/Go coupled GPCR. Upon activation of CMKLR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GRPR Activated GPCR Internalization Assay, 2-Plate Kit. (93-0667E3CP5M)
The PathHunter GRPR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GRPR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged GRPR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting GRPR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR5 Activated GPCR Internalization Assay, 2-Plate Kit. (93-0653E3CP0M)
The PathHunter CCR5 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCKAR Activated GPCR Internalization Assay, 2pl Kit. (93-0650E3CP5M)
The PathHunter CCKAR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCKAR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCKAR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCKAR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter PL/PK Detection kit, 4,000 dp (384-well). (93-0812)
The PathHunter ProLabel/PK Detection kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 10 plates (96/384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0812.
Eurofins Discoverx PathHunter eXpress OPRK1 Total Internalization Assay, 10pl Kit. (93-0828E3CP0L)
The PathHunter OPRK1 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OPRK1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged OPRK1 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRK1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc CytoSelect™ 24-Well Cell Invasion Assay Kit (Collagen I, Colorimetric), 12 assays
Cell invasion is the ability of malignant tumor cells to invade normal surrounding tissue, which contributes in large part to the significant morbidity and mortality of cancers. The CytoSelect™ Cell Invasion Assay Kit utilizes polycarbonate membrane inserts (8 µm pore size) to assay the invasive properties of cells. The kit contains twelve 8 um polycarbonate membrane inserts coated with a Collagen gel layer in a 24-well plate. Cells must degrade the Collagen layer and then move through the pores of the membrane. Invasive cells are then quantified using the provided Cell Stain Solution.
Amsbio LLC Heparanase Assay Kit without Positive Control Enzyme
Measure enzyme activity of heparanase in cell culture supernatants, human plasma, biological fluids and tissue samples. These kits provide a solution to the performance and standardization problems found with traditional measurement techniques used for these types of enzymes.
Molecular Devices CatchPoint cGMP Assay Kit, 384-Well, Clear Bottom
The CatchPoint cGMP Fluorescent Assay Kit Explorer configuration is a high-sensitivity, cell-compatible immunoassay for quantitative measurement of cyclic GMP in high-throughput formats. The kit provides pre-coated 384-well plates and optimized reagents for detecting low cGMP levels with a stable fluorescent readout and flexible timing for automation.
- Pre-coated, pre-blocked 384-well black well, clear bottom plates, two plates per kit
- Includes reagents and buffers for assay setup and detection, including antibody, conjugate, calibrator, and substrate
- Fluorescent Stoplight Red readout with stable signal from 10 minutes up to 24 hours
- Quantitative competitive immunoassay format suitable for cell-based assays and HTS
- Robust format resists interference from colored or fluorescent test compounds
Molecular Devices Reporter Assay Kit, Dual Luciferase, 1,000 Reactions
SpectraMax DuoLuc Reporter Assay Kit enables sensitive, quantitative measurement of firefly and Renilla luciferase activities in mammalian cells using flash-type luminescence. The bulk format supports high-throughput workflows and is optimized for use with microplate readers equipped with injectors. Preconfigured reader protocols simplify data acquisition and normalization to internal controls.
- Dual luciferase assay for firefly and Renilla measurements.
- Bulk kit provides reagents for 1,000 reactions.
- Flash-type luminescence chemistry for high sensitivity.
- Optimized for microplate readers with injector capability.
- Enables normalization to an internal control reporter.
- Includes assay reagents; additional passive lysis buffer available separately.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Advanced Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-ERK (Thr202/Tyr204) Advanced Detection Kit, 10,000 Assay Points